Special Collections Procedural Competency

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Course Objectives

Explain the significance of a peripheral blood smear, bleeding time test, PRP & PRF treatments.
Demonstrate technical proficiency in peripheral blood smear preparation, bleeding time testing, and PRP/PRF processing.
Recognize and avoid pre-analytical errors that may affect test accuracy as well as patient outcomes.
Ensure compliance with regulatory standards, safety protocols, and ensure bloodborne pathogen standards are followed.
Identify clinical applications and emerging uses of PRP and PRF in multidisciplinary settings.

Course Content

Course Creator: Nichelle Volker RMA

Total CE Hours: 1 Credit Hour

Pace Course #: 586-2410-25

Key Terms

Aggregation
Buffy coat
Diff-Safe
Feathered-edge
Fibrin degradation product
Hemolyze/ Hemolysis
Hemostasis
Icteric
Lipemic
Nosocomial infection
Phagocytic
Platelets
Red Blood Cell
Vasoconstriction
Volar
von Willebrand factor
White Blood Cells

Outline:

Introduction
Peripheral Blood Smear
- Whole blood
  - Plasma
  - RBC
  - WBC
  - Platelets
- Define PBS
  - Why it is done/what it can diagnose
- Common Issues and solutions
- Supplies for PBS
- Creating a PBS
- Supplies for capillary method creation of PBS
- Creating a PBS via capillary collection method
Bleeding Time Test
- Define BTT
  - Why it is done/what it can diagnose
- Hemostasis overview
Platelet Function
- Bleeding Disorders
BTT less commonly used
Testing Methods
- IVY
  - Supplies
  - Steps
- DUKE
  - Supplies
  - Steps
PRP & PRF
- Define
  - Why it is done/where it is used
    - Treatment types
  - Classifications
- Centrifugation techniques
- PRP Procedure
  - Supples
  - PRP method
  - Buffy coat method
- PRF Procedure
  - PRF supplies
  - Choukroun’s original protocol
  - Steps for L-PRF
  - Steps for I-PRF
  - Steps for C-PRF
- Safety precautions
Compliance and Safety
- OSHA guidelines
  - Lab rules
  - Patient care area rules
  - BBP Standard
  - Exposure Control Plan
  - Education and Training
  - Hepatitis B Vaccine
Conclusion

References:

Blood smear. (2025, February 19). In Wikipedia. https://en.wikipedia.org/wiki/Blood_smear
Anderson , G. R. (2022). A FUNDAMENTAL GUIDE TO PHLEBOTOMY (E. K. Eaton, Ed.) (p. 46). NHCO – National Healthcare Certification Organization. https://instructors.phlebotomyusa.com/s3_preview/uploads/specifications/3559/2023-pts-textbook.pdf
Hadidi, S. A. (2021, August 17). Blood Smear: The Fifth Vital Sign in Hematology. Hematology.org. https://www.hematology.org/education/trainees/fellows/hematopoiesis/2021/blood-smear#:~:text=Make%20sure%20the%20smear%20was,be%20needed%20for%20proper%20interpretation.
Phagocyte. Britannica. Retrieved June 19, 2025, from https://www.britannica.com/science/phagocyte
Bleeding time. (2024, October 1). In Wikipedia. https://en.wikipedia.org/wiki/Bleeding_time#:~:text=A%20lancet%20or%20scalpel%20blade,is%20called%20the%20bleeding%20time.
Dr Choukroun Instrument Set – PRF Group Corp. (n.d.). https://prfgroupcorp.com/store/prf-choukroun/prf/kit-of-prf-instruments/prf-instrument-kit/
Mito Kobayashi, Tomoyuki Kawase, Makoto Horimizu, Kazuhiro Okuda, Larry F. Wolff, Hiromasa Yoshie, A proposed protocol for the standardized preparation of PRF membranes for clinical use, Biologicals, Volume 40, Issue 5, 2012, Pages 323-329, ISSN 1045-1056, https://doi.org/10.1016/j.biologicals.2012.07.004.
Miron, Richard & Fujioka-Kobayashi, Masako & Zhang, Yufeng. (2021). Protocols for PRF.
Dashore, S., Chouhan, K., Nanda, S., & Sharma, A. (2021). Platelet-Rich Fibrin, Preparation and Use in Dermatology. Indian dermatology online journal, 12(Suppl 1), S55–S65. https://doi.org/10.4103/idoj.idoj_282_21