PRP and PRF: Specialty Clinical Applications

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Course Objectives
Identify the origins of PRP and PRF 
Differentiate PRP from PRF
Discuss the various clinical applications
Demonstrate how to prepare PRP and PRF
Course Content
Course Creator: Nichelle Volker RMA
Total CE Hours: 1 Credit Hour 
Pace Course #: 586-2409-25
Outline:
  • Whole blood and hemostasis
    • What is whole blood
      • Components
      • Percentages
    • What is hemostasis
      • 4 stages of hemostasis
      • The coagulation cascade
    • What is PRF?
  • History of PRP & PRF
    • PRP History
      • Timeline of scientific development and discoveries
      • Discoveries of growth factors
    • PRF History
      • Second generation to PRP
      • Drawbacks of PRP that led to PRF
      • Choukroun
  • Differentiating PRP from PRF
    • Classifications
      • P-PRP
      • L-PRP
      • P-PRF
      • L-PRF
      • I-PRF
    • Vendiagram comparison
  • Preparation of PRP and PRF
    • Safety guidelines
    • PRP preparation
      • Supplies
      • PRP method
      • Buffy-coat method
    • PRF prepatation
      • Supplies
      • Variations and non-standardized protocol
      • Choukroun’s original preparation
      • 3 dominant preparation methods
        • L-PRF
        • I-PRF
        • C-PRF
  • Applications of PRP and PRF
    • Clinical settings
    • Treatments
      • Transfusion
      • Cardiology
      • Dental
      • Sports medicine
      • Orthopedic
      • Gynecology
      • Dermatology
      • Aesthetic spa and salon treatments
      • Micro needling vs nano needling
  • Conclusion 

References: 
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  2. Anderson, G. (2022). A Fundamental Guide to Phlebotomy: a competency based approach (E. Kammer Eaton, Ed.; First Edition). National Healthcare Certification Organization. 
  3. LaPelusa, A., & Dave, H. D. (2023). Physiology, Hemostasis. In StatPearls. StatPearls Publishing.
  4. Coagulation - intrinsic - extrinsic - fibrinolysis - TeachMePhysiology. (2023, July 17). TeachMePhysiology. https://teachmephysiology.com/immune-system/haematology/coagulation/
  5. Sunitha Raja, V., & Munirathnam Naidu, E. (2008). Platelet-rich fibrin: evolution of a second-generation platelet concentrate. Indian journal of dental research : official publication of Indian Society for Dental Research, 19(1), 42–46. https://doi.org/10.4103/0970-9290.38931
  6. Mościcka, P., & Przylipiak, A. (2021). History of autologous platelet-rich plasma: A short review. Journal of cosmetic dermatology, 20(9), 2712–2714. https://doi.org/10.1111/jocd.14326
  7. Pavlovic, V., Ciric, M., Jovanovic, V., Trandafilovic, M., & Stojanovic, P. (2021). Platelet-rich fibrin: Basics of biological actions and protocol modifications. Open medicine (Warsaw, Poland), 16(1), 446–454. https://doi.org/10.1515/med-2021-0259
  8. Platelet-Rich plasma (PRP) injections. (2023, June 9). Johns Hopkins Medicine. https://www.hopkinsmedicine.org/health/treatment-tests-and-therapies/plateletrich-plasma-prp-treatment#:~:text=Studies%20show%20that%20the%20increased,and%20even%20encouraging%20hair%20growth. 
  9. Dohan Ehrenfest, D. M., Andia, I., Zumstein, M. A., Zhang, C. Q., Pinto, N. R., & Bielecki, T. (2014). Classification of platelet concentrates (Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications and perspectives. Muscles, ligaments and tendons journal, 4(1), 3–9.
  10. Diab, N. A. F., Ibrahim, A. M., & Abdallah, A. M. (2023). Fluid Platelet-Rich Fibrin (PRF) Versus Platelet-Rich Plasma (PRP) in the Treatment of Atrophic Acne Scars: A Comparative Study. Archives of dermatological research, 315(5), 1249–1255. https://doi.org/10.1007/s00403-022-02511-3
  11. Dr Choukroun Instrument Set – PRF Group Corp. (n.d.). https://prfgroupcorp.com/store/prf-choukroun/prf/kit-of-prf-instruments/prf-instrument-kit/
  12. Mito Kobayashi, Tomoyuki Kawase, Makoto Horimizu, Kazuhiro Okuda, Larry F. Wolff, Hiromasa Yoshie, A proposed protocol for the standardized preparation of PRF membranes for clinical use, Biologicals, Volume 40, Issue 5, 2012, Pages 323-329, ISSN 1045-1056, https://doi.org/10.1016/j.biologicals.2012.07.004.
  13. Miron, Richard & Fujioka-Kobayashi, Masako & Zhang, Yufeng. (2021). Protocols for PRF. 
  14. Dashore, S., Chouhan, K., Nanda, S., & Sharma, A. (2021). Platelet-Rich Fibrin, Preparation and Use in Dermatology. Indian dermatology online journal, 12(Suppl 1), S55–S65. https://doi.org/10.4103/idoj.idoj_282_21 
  15. Asherman's Syndrome. (2025, March 19). Cleveland Clinic. https://my.clevelandclinic.org/health/diseases/16561-ashermans-syndrome
  16. Dawood, A. S., & Salem, H. A. (2018). Current clinical applications of platelet-rich plasma in various gynecological disorders: An appraisal of theory and practice. Clinical and experimental reproductive medicine, 45(2), 67–74. https://doi.org/10.5653/cerm.2018.45.2.67
  17. Bertrand, A. (2024, April 27). Microneedling vs Nanoneedling — Just Skin Dermatology. Just Skin Dermatology. https://www.justskin.co/blog/microneedling-vs-nanoneedling

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